A Contraction Theory Approach to Singularly Perturbed Systems with Application to Retroactivity Attenuation Citation

In this paper, we revisit standard results for singularly perturbed systems on the infinite time interval by employing tools from nonlinear contraction theory. This allows us to determine explicit bounds both on the rate of convergence of trajectories to the slow manifold, and on the distance between these trajectories and those of the reduced system. We illustrate the application of the proposed technique to the problem of retroactivity attenuation in biomolecular systems, that is, to the problem of attenuating the effects of output loading due to interconnection to downstream systems. By virtue of the explicit bounds, we can single out the key biochemical parameters to tune in order to enhance retroactivity attenuation. This provides design guidelines for synthetic biology devices that are robust to loading and can function as insulation devices just like insulating amplifiers work in electronics.